Antioxidant Activity of a PopularFormulation Claimed By The TraditionalHerbal Medicine Practitioners ofPuducherry To Be Effective In ProphylaxisAnd Therapeutic Management Of Covid-19

Abstract

The aim of this research work is to evaluate the antioxidant activity of the hydroalcoholic extract of herbal formulation for COVID-19 by in silico and in vitro approach. The reported phytoconstituents of ingredients of herbal formulation were explored for molecular docking using Autodock Vina against the four targeted proteins such as lipoxygenase, NADPH oxidase, myeloperoxidase and xanthine oxidase. In vitro antioxidant activity was evaluated by DPPH radical scavenging and Hydroxyl Radical scavenging assay. The molecular docking study revealed that all phytoconstituents exhibited negative binding energy which indicates that they possess inhibitory activity. Among 157 phytoconstituents, LicoricesaponinA3 showed least binding energies such as12.3Kcal/mol, -11.9Kcal/mol and -11.4Kcal/mol for the protein myeloperoxidase, NADPH oxidase, and xanthine oxidase respectively and 19-acetoxy-7, 9, 10 deacetyl-baccin VI showed least binding energy (-11.2Kcal/mol) for the protein lipoxygenase. The in vitro antioxidant activity revealed that the hydroalcoholic extract showed dose dependent scavenging activity and maximum activity at 1mg/ml concentration. Based on the results obtained it is concluded that herbal formulation possesses antioxidant activity