Antioxidant Activity of a PopularFormulation Claimed By The TraditionalHerbal Medicine Practitioners ofPuducherry To Be Effective In ProphylaxisAnd Therapeutic Management Of Covid-19

Abstract

The aim of this research work is to
evaluate the antioxidant activity of the
hydroalcoholic extract of herbal formulation for
COVID-19 by in silico and in vitro approach. The
reported phytoconstituents of ingredients of herbal
formulation were explored for molecular docking
using Autodock Vina against the four targeted
proteins such as lipoxygenase, NADPH oxidase,
myeloperoxidase and xanthine oxidase. In vitro
antioxidant activity was evaluated by DPPH radical
scavenging and Hydroxyl Radical scavenging assay.
The molecular docking study revealed that all
phytoconstituents exhibited negative binding
energy which indicates that they possess inhibitory
activity. Among 157 phytoconstituents, LicoricesaponinA3 showed least binding energies such as12.3Kcal/mol, -11.9Kcal/mol and -11.4Kcal/mol
for the protein myeloperoxidase, NADPH oxidase,
and xanthine oxidase respectively and 19-acetoxy-7,
9, 10 deacetyl-baccin VI showed least binding
energy (-11.2Kcal/mol) for the protein
lipoxygenase. The in vitro antioxidant activity
revealed that the hydroalcoholic extract showed
dose dependent scavenging activity and maximum
activity at 1mg/ml concentration. Based on the
results obtained it is concluded that herbal
formulation possesses antioxidant activity