In Vitro Anthelminthic activity Of Herbal Formulation Against Eisenia fetida
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Abstract
The present study was undertaken to evaluate anthelmintic activity of Herbal formulation of leaves of Platyclatus orientalis, Momordica charantia and Punica granatum against Eisenia fetida. For this study used various concentrations (25-100 mg/ml) herbal formulation were evaluated in the bioassay involving determination of time of paralysis (P) and time of death (D) of the worms. Albendazole was used as standard anthelmintic drug and distilled water was used as control. The results of present study indicated that the herbal formulation significantly exhibited paralysis (P<0.01) in worms in higher dose 100 mg/ml and also caused death of worms especially at higher concentration of 100 mg/ml, as compared to standard drug.
References
Bundy D. A. (1994). Trans Royal Soc Trop Med Hyg, 88: 259–261.
Tagbota S., Townson S. (2001). Adv Parasitol, 50: 199–205.
Sondhi S. M., Shahu R., Magan Archana. (1994). Indian Drugs, 31(7): 317–320.
Srivastava P., Kumar P., Singh D. K., Singh V. K. (2012). Biological Properties of Thuja orientalis Linn. Advan. Life Sci., 2(2): 17–20.
Chizzola R., Hochsteiner W., Hajek S. (2004). GC analysis of essential oils in the rumen fluid after incubation of Thuja orientalis twigs in the Rusitec system. Res. Vet. Sci., 76(1): 77–82.
Breeta R. E., Jesubatham P. D., Grace V. M. B., Viswanathan S., Srividya S. (2018). Non-toxic and non-teratogenic extract of Thuja orientalis L. inhibited angiogenesis in zebra fish and suppressed the growth of human lung cancer cell line. Biomed. Pharmaco., 106: 699–706.
Vashista P. C. (1974). Taxonomy of Angiosperms. 2nd ed., R. Chand and Co., New Delhi.
Nayar N. M., Singh R. (1998). Taxonomy, distribution and ethnobotanical uses. In: Nayar & More (Eds.), Cucurbits. Academic Press, New York, pp. 1–18.
Sastri B. N. (1962). Momordica charantia. Wealth of India – Raw Materials, 6: 51–54.
Lee-Hung S., Hung P. L., Chen H. C., Bourinbaiar A., Hung H. I., Kung H. F. (1995). Anti-HIV and antitumor activity of recombinant MAP-30 from bitter melon. Gene, 161: 151–156.
Olapour S., Najafzadeh H. (2010). Evaluation of analgesic, anti-inflammatory and antiepileptic effect of hydro-alcoholic peel extract of Punica granatum. Asian J. Med. Sci., 2: 266–270.
Ahmad I., Beg A. Z. (2001). Antimicrobial and phytochemical studies on 45 Indian medicinal plants against multi-drug resistant human pathogens. J. Ethnopharmacol., 74: 113–133.
Naz S., Siddiqi R., Ahmad S., Rasool S. A., Sayeed S. A. (2007). Antibacterial activity-directed isolation of compounds from Punica granatum. J. Food Sci., 72: 341–345.
Shan B., Cai Y. Z., Brooks J., Corke H. (2007). In-vitro antibacterial activity of dietary spices and medicinal herb extracts. Int. J. Food Microbiol., 117: 112–119.
Cowan M. M. (1999). Plant products as antimicrobial agents. Clin. Microbiol. Rev., 12: 564–582.
Braga L. C., Shupp J. W., Cummings C., et al. (2005). Pomegranate extract inhibits Staphylococcus aureus growth and enterotoxin production. J. Ethnopharmacol., 96: 335–339.
Reddy M., Gupta S., Jacob M., Khan S., Ferreira D. (2007). Antioxidant, antimalarial and antimicrobial activities of tannin-rich fractions from Punica granatum. Planta Medica, 73: 461–467.
Sajjad W., Ilahi N., Hayat M., Ahmad F., Rahman Z. U. (2015). Phytochemical screening and antitumor potential of Punica granatum peel extract. Int. J. Biosci., 7: 102–110.
Lama Y. C., Ghimire S. K., Thomas Y. A. (2001). Medicinal plants of Dolpo. WWF Nepal Program, Kathmandu.
Holetz F. B., Pessini G. L., Sanches N. R., et al. (2002). Screening of Brazilian folk medicinal plants for infectious diseases. Mem. Inst. Oswaldo Cruz, 97: 326–332.
Dash G. K., Mishra B., Panda A., Patro C. P., Ganapaty S. (2003). Anthelmintic activity of Evolvulus nummularius. Indian J. Nat. Prod., 19(3): 24.
Tambe V. D., Nirmal S. A., Jadhav R. S., et al. (2006). Anthelmintic activity of Wedelia trilobata leaves. Indian J. Nat. Prod., 22: 27–29.
Mali R. G., Mahajan S. G., Mehta A. A. (2007). In-vitro anthelmintic activity of Mimusops elengi stem bark. PHCOG MAG., 3(10): 73–76.
Martin R. J. (1985). GABA and piperazine-activated currents in Ascaris suum. Br. J. Pharmacol., 84(2): 445–461.
Szewezuk V. D., Mongelli E. R., Pomillo A. B. (2003). Antiparasitic activity of Melia azadirach. Mole. Med. Chem., 1: 54–57.
Mali R. G., Mahajan S., Patil K. S. (2005). Anthelmintic activity of Capparis spinosa root bark. Indian J. Nat. Prod., 21(4): 50–51.
Hogade M. G., Patil K. S., Jalalpure S. S., et al. (2009). Anthelmintic activity of Morus alba fruit. J. Pharmaceutical Sciences, 2(1): 28–31.
Shivkar Y. M., Kumar V. L. (2003). Anthelmintic activity of Calotropis procera latex. Pharm. Biol., 41(4): 263–265.
Athnasiadou S., Kyriazakis F., Jackson R. L., Coop R. L. (2001). Direct anthelmintic effects of condensed tannins. Vet. Parasitol., 99: 19.
Thompson D. P., Geary T. G. (1995). In: Marr J. J. (Ed.), Biochemistry and Molecular Biology of Parasites. Academic Press, New York.
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