an in-Depth study is conducted on the segmented Genes of the Cancer Causing Helicobacter pylori

Abstract

Helicobacter Pylori is a kind of bacterium  that, when present in human bodies, has the potential to  cause persistent gastritis. The inner lining of the stomach  can become inflamed, a condition known as gastritis.  It is also possible that this bacterium has a part in the  formation of stomach ulcers and stomach cancer. At  first, this bacteria did not cause any harm and behaved  exactly as one would anticipate it would. However, as  a result of certain alterations in the structure of the  genome (a process that is known as genetic mutation),  it turned out to be dangerous. To this day, researchers  have pinpointed 27 distinct gene alterations that, if left  unchecked, might eventually result in the development of  cancer. These genes have numbers that vary from HP0821  all the way up to HP0847 connected with them. Finding  out what had occurred to cause the shift in those 27  genes was the most significant objective that we aimed to  achieve with our thesis research. The algorithm that we  conceived of and put into action for this specific objective  was developed with the help of the Java programming  language. This function receives its input from a single file  and generates a total of six additional files as its output.  We make changes to the nucleotide sequences of 27 genes  in order to zero in on the specific location in the genome  where mutations took happened. After that, we made use  of a program called BLAST to look for genes in other  bacteria that were similar to the ones we were looking at.  We discovered that H. pylori had the highest similarity,  which came in at a whopping 100%. In addition, we  found a similarity of 90% with the D. desulfuricans  ND132 chromosome as well as 85% with the Aeromonas  hydrophila SSU genomic scaffold supercont1.1.